Similarity:
Both prokaryotes and eukaryotes have genes that encode proteins and other functional RNA molecules. These genes are transcribed into RNA molecules, which are then translated into proteins in both types of organisms.
Difference:
One major difference in gene organization between prokaryotes and eukaryotes is the presence of introns in eukaryotic genes. Prokaryotic genes lack introns, whereas eukaryotic genes often contain introns that are removed during RNA processing. This difference in intron-exon structure affects the complexity and regulation of gene expression between the two types of organisms